High expression of acidic chitinase and chitin digestibility in the stomach of common marmoset (Callithrix jacchus), an insectivorous nonhuman primate.

Chitin is a polymer of N-acetyl-D-glucosamine (GlcNAc) and the main constituent of the exoskeleton of insects. The insects are rich in protein with high energy conversion efficiency. More recently, we have reported that the chitinase acid (Chia) acts asMouse Clia Kits  a digestive enzyme in mice and swine (omnivorous) but not in dogs (carnivores) and bovine (herbivores), showed that eating behavior affects the level of expression of Chia, and determine the digestibility of chitin in certain animals. Common marmosets (Callithrix jacchus) belongs to the family of New World monkeys and provide a potential bridge between mouse models and human diseases. 

Common marmosets are non-human primates insect with an unknown level of expression and enzymatic function of homologous Chia, Chia. Here, we report that the common marmoset are very revealing pepsin-, trypsin- and chymotrypsin CHIA hold in the stomach. We show that the CHIA most active at pH 2.0 and degradation of chitin and shellfish mealworm to GlcNAc dimers in gastrointestinal conditions. 

Despite the common marmoset and crab-eating monkeys (Old World monkeys) have two CHIA gene in their genome, they are particularly revealing of the genes in the stomach. Thus, this study is the first to investigate the level of expression and enzymatic functions CHIA in New World primates, contribute to the understanding of adaptation andgentaur.us Ovine Clia Kits digestion of food in this taxon

Using the advantages of phenol red, the signal enhancer, and bovine serum albumin (BSA), stabilizer of horseradish peroxidase (HRP), was added in the reaction of Amplex Red HRP enzyme and H2O2, 1,1'-oxalyldiimidazole highly sensitive chemiluminescence enzyme immunoassay (ODI- CLEIA) was developed to quantify the trace level quickly carcinoembryonic antigen (CEA) in human serum. Phenol red acts as an enhancer on ODI-CLEIA while BSA supported steady and rapid activation of HRP. CL emission from resorufin formed from HRP enzyme reaction in the presence of BSA and phenol red about 70 times brighter than the absence of both materials. 

ODI-CLEIA in the presence of BSA (1.5 mg / ml) .and phenol red (1 mM) were able to quickly analyze CEA in human serum with a wide linear calibration curve (2.5 to 100 ng / ml). The limit of detection (LOD = 3σ / slope) of the ODI-CLEIA is as low as 0.19 ng / ml. In addition, it was confirmed that the accuracy, precision, and reproducibility of ODI-CLEIA in the presence of BSA and phenol red was good with a range of statistical error is acceptable.